Microculture Plaque Neutralization Test for California Group Arboviruses

Kinetics of the vaccinia virus plaque neutralization test.

Neutralization of vaccinia virus with immune rabbit serum occurred optimally when incubated at 37 C for 16 to 24 hr in the plaque neutralization test employing the MA-104 embryonic rhesus monkey kidney cell line.

Varicella-zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique.

A new plaque assay for the quantitation of varicella-zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed using the indirect immunoperoxidase technique. As compared with the classical plaque assay using a solid overlay, the test gives earlier results since plaque counting can be performed on day 3 after the inoculation ...

Interference among group A arboviruses.

Interference among group A arboviruses is described which does not involve the mediation of interferon. Interference was observed only if the interfering virus had an advantage over the challenge virus, either in time or in multiplicity of infection. Adsorption, penetration, and uncoating of challenge virus did not appear to be inhibited, but the synthesis of infectious viral ribonucleic acid o...

Immunological overlap and identification of group A arboviruses by a gradient plate plaque technique.

A semi-quantitative, gradient plate, plaque-neutralization technique was developed for studying immunological overlap and identification of Group A arboviruses. Two principles of the technique are: (i) a concentration gradient of antibody is provided that continues into a control area without antibody, and (ii) neutralization is measured by inhibition of plaque formation in agar-cell suspension...

Comparison of Micro Neutralization Test with Hemadsorption-Inhibition Test, and Immunoflrorescence Technique for Detecting Mumps Antibodies